Chromatin Structure & Function Lab

Schalch Lab Website

Structure Gallery

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Chp2 chromoshadow domain in complex with N-terminal domain of chromatin remodeler Mit1

PDBID: 6FTO

Leopold K., Stirpe A. and Schalch, T. (2019). Transcriptional gene silencing requires dedicated interaction between HP1 protein Chp2 and chromatin remodeler Mit1. Genes & Development, Published in Advance, doi: 10.1101/gad.320440.11. Open Access Article

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Crystal structure of the DNA binding domain of fission yeast Sap1

PDBIDs: 6EXU (dimethylarsenic adduct) and 6EXT (native)
Jorgensen et al. (2017). Structure of the replication regulator Sap1 reveals functionally important interfaces. (2018) Sci Rep 8: 10930-10930. Open Access Article

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Structures of the 4_601_157 tetranucleosome

PDBID: 5OY7, PDBID: 5OXV

Ekundayo, B., Richmond, T.J., and Schalch, T. (2017). Capturing structural heterogeneity in chromatin fibers. Journal of Molecular Biology 429, 3031-3042. Article, UNIGE archives

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Structure of the histone deacetylase Clr3

PDBID: 5IKK

Job, G.*, Brugger, C.*, Xu, T.*, Lowe, B.R.*, Pfister, Y., Qu, C., Shanker, S., Baños Sanz, J.I., Partridge, J.F. † and Schalch, T. † (2016). SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules. Molecular Cell, 62, 207–221. Article

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Structure of Clr2 bound to the Clr1 C-terminus

PDBID: 5IKJ

Job, G.*, Brugger, C.*, Xu, T.*, Lowe, B.R.*, Pfister, Y., Qu, C., Shanker, S., Baños Sanz, J.I., Partridge, J.F. † and Schalch, T. † (2016). SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules. Molecular Cell, 62, 207–221. Article

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Crystal structure of the C-terminal domain of the Mit1 nucleosome remodeler in complex with Clr1

PDBID: 5IKF

Job, G.*, Brugger, C.*, Xu, T.*, Lowe, B.R.*, Pfister, Y., Qu, C., Shanker, S., Baños Sanz, J.I., Partridge, J.F. † and Schalch, T. † (2016). SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules. Molecular Cell, 62, 207–221. Article

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Structure of Dos1 β-propeller

PDBID: 4O9D

Kuscu, C., Zaratiegui, M., Kim, H.S., Wah, D.A. Martienssen, R.A., Schalch, T.† and Joshua-Tor, L.†(2014). CRL4-like Clr4 Complex in Schizosaccharomyces Pombe Depends on an Exposed Surface of Dos1 for Heterochromatin Silencing. PNAS, 111, 1795-1800. Article

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Crystal structure of the Chp1-Tas3 complex core

PDBID: 3TIX

Schalch, T., Job, G., Shanker, S , Partridge, J. F., Joshua-Tor, L. (2011). The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Nature Structural and Molecular Biology 18, 1351–7. Article

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Chromodomain of Chp1 in complex with Histone H3K9me3 peptide

PDBID: 3G7L

Schalch, T.*, Job, G.*, Noffsinger, V. J.*, Shanker, S., Kuscu, C., Joshua-Tor, L., and Partridge, J. F. (2009). High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin. Molecular Cell 34, 36–46. Article

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Structure of the 4_601_167 Tetranucleosome

PDBID: 1ZBB

Schalch, T., Duda, S., Sargent, D. F., and Richmond, T. J. (2005). X-ray structure of a tetranucleosome and its implications for the chromatin fibre. Nature 436, 138–41. Article