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Structure Gallery

Structure of Clr4 mutant – F256A/F310A/F427A bound to SAH

PDBID: 6Z2A

Stirpe A., Guidotti N., Northall S., Kilic, S., Hainard, A., Vadas, O., Fierz, B., Schalch, T. (preprint). SUV39 SET domains mediate crosstalk of heterochromatic histone marks. eLife 10, e62682, Open Access Article, bioRxiv Preprint

Chp2 chromoshadow domain in complex with N-terminal domain of chromatin remodeler Mit1

PDBID: 6FTO

Leopold K., Stirpe A. and Schalch, T. (2019). Transcriptional gene silencing requires dedicated interaction between HP1 protein Chp2 and chromatin remodeler Mit1. Genes & Development, Published in Advance, doi: 10.1101/gad.320440.11. Open Access Article

Crystal structure of the DNA binding domain of fission yeast Sap1

PDBIDs: 6EXU (dimethylarsenic adduct) and 6EXT (native)

Jorgensen et al. (2017). Structure of the replication regulator Sap1 reveals functionally important interfaces. (2018) Sci Rep 8: 10930-10930. Open Access Article

Structures of the 4_601_157 tetranucleosome

PDBID: 5OY7, PDBID: 5OXV

Ekundayo, B., Richmond, T.J., and Schalch, T. (2017). Capturing structural heterogeneity in chromatin fibers. Journal of Molecular Biology 429, 3031-3042. Article, UNIGE archives

Structure of the histone deacetylase Clr3

PDBID: 5IKK

Job, G.*, Brugger, C.*, Xu, T.*, Lowe, B.R.*, Pfister, Y., Qu, C., Shanker, S., Baños Sanz, J.I., Partridge, J.F. † and Schalch, T. † (2016). SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules. Molecular Cell, 62, 207–221. Article

Structure of Clr2 bound to the Clr1 C-terminus

PDBID: 5IKJ

Job, G.*, Brugger, C.*, Xu, T.*, Lowe, B.R.*, Pfister, Y., Qu, C., Shanker, S., Baños Sanz, J.I., Partridge, J.F. † and Schalch, T. † (2016). SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules. Molecular Cell, 62, 207–221. Article

Crystal structure of the C-terminal domain of the Mit1 nucleosome remodeler in complex with Clr1

PDBID: 5IKF

Job, G.*, Brugger, C.*, Xu, T.*, Lowe, B.R.*, Pfister, Y., Qu, C., Shanker, S., Baños Sanz, J.I., Partridge, J.F. † and Schalch, T. † (2016). SHREC Silences Heterochromatin via Distinct Remodeling and Deacetylation Modules. Molecular Cell, 62, 207–221. Article

Structure of Dos1 β-propeller

PDBID: 4O9D

Kuscu, C., Zaratiegui, M., Kim, H.S., Wah, D.A. Martienssen, R.A., Schalch, T.† and Joshua-Tor, L.†(2014). CRL4-like Clr4 Complex in Schizosaccharomyces Pombe Depends on an Exposed Surface of Dos1 for Heterochromatin Silencing. PNAS, 111, 1795-1800. Article

Crystal structure of the Chp1-Tas3 complex core

PDBID: 3TIX

Schalch, T., Job, G., Shanker, S , Partridge, J. F., Joshua-Tor, L. (2011). The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS. Nature Structural and Molecular Biology 18, 1351–7. Article

Chromodomain of Chp1 in complex with Histone H3K9me3 peptide

PDBID: 3G7L

Schalch, T.*, Job, G.*, Noffsinger, V. J.*, Shanker, S., Kuscu, C., Joshua-Tor, L., and Partridge, J. F. (2009). High-affinity binding of Chp1 chromodomain to K9 methylated histone H3 is required to establish centromeric heterochromatin. Molecular Cell 34, 36–46. Article

Structure of the 4_601_167 Tetranucleosome

PDBID: 1ZBB

Schalch, T., Duda, S., Sargent, D. F., and Richmond, T. J. (2005). X-ray structure of a tetranucleosome and its implications for the chromatin fibre. Nature 436, 138–41. Article