Unlocking the Secrets of Genome Regulation using cryo-EM
Are you an ambitious spirit, ready to make breakthrough discoveries at the frontier of epigenetics? Dive deep into the fascinating world of eukaryotic genomes with us!
Here is what awaits you:
A Deep Dive into Chromatin: Become an expert on the intricate structure of chromatin and its effector complexes. These vital components steer transcription, genome maintenance, repair, and replication.
Zoom in on Histone Ubiquitination: This project is your chance to become immersed in the questions surrounding histone ubiquitination. Histone proteins coat the genomes of eukaryotic organisms, serving as a signalling platform vital for genome regulation and protection. Through the study of “writer”, “eraser”, and “reader” proteins, you’ll explore the mechanisms that shape chromatin, the structure of life’s program.
Hands-on Techniques and Real-World Experience: Dive into techniques spanning biochemistry, structural biology, genome analysis, and cell biology. Explore the structure and function of the H2B ubiquitin ligase complex, gaining invaluable experience in the lab and sharpening your skills to uncover the core of biological processes.
Be at the Forefront of a Thrilling Field: This is more than just a project; it’s a golden intellectual opportunity. Immerse yourself in a rapid-paced, competitive field that promises challenges and rewards.
International Collaboration and Cutting-Edge Tools: You won’t be on this journey alone! This initiative is part of a collaborative push, tapping into our strength in high-resolution structural analysis. You’ll have the chance to use advanced tools like cryo-EM to visualize the sophisticated cellular machinery driving ubiquitination.
Creating a Lasting Impact: By establishing structure-function relationships and integrating structural insights with biochemistry and genetic analysis, we strive to unearth principles that steer eukaryotic genome regulation. Ultimately, this knowledge may contribute to therapeutic innovations.
Techniques that may be undertaken during the project
Cryo-EM, Alphafold II modelling, cloning, protein expression and purification from insect cells and bacteria, enzymatic assays, biophysical techniques. S. pombe genetics, Chromatin Immunoprecipitation (ChIP-seq), RT-qPCR
Apply now!
Application page: https://warwick.ac.uk/fac/cross_fac/mibtp/phd/application